http://repository.vnu.edu.vn/handle/VNU_123/33080 |
Thứ Sáu, 26 tháng 5, 2017
Targeting specificity of dendritic cells on breast cancer stem cells: in vitro and in vivo evaluations
Breast cancer is a leading cause of death in women, and almost all complications are due to chemotherapy resistance. Drug-resistant cells with stem cell phenotypes are thought to cause failure in breast cancer chemotherapy. Dendritic cell (DC) therapy is a potential approach to eradicate these cells. This study evaluates the specificity of DCs for breast cancer stem cells (BCSCs) in vitro and in vivo. BCSCs were enriched by a verapamil-resistant screening method, and reconfirmed by ALDH expression analysis and mammosphere assay. Mesenchymal stem cells (MSCs) were isolated from allogeneic murine bone marrow. DCs were induced from bone marrow-derived monocytes with 20 ng/mL GC-MSF and 20 ng/mL IL-4. Immature DCs were primed with BCSC-or MSC-derived antigens to make two kinds of mature DCs: BCSCDCs and MSC-DCs, respectively. In vitro ability of BCSC-DCs and MSC-DCs with cytotoxic T lymphocytes (CTLs) to inhibit BCSCs was tested using the xCELLigence technique. In vivo, BCSC-DCs and MSC-DCs were transfused into the peripheral blood of BCSC tumor-bearing mice. The results show that in vitro BCSC-DCs significantly inhibited BCSC proliferation at a DC: CTL ratio of 1: 40, while MSC-DCs nonsignificantly decreased BCSC proliferation. In vivo, tumor sizes decreased from 18.8% to 23% in groups treated with BCSC-DCs; in contrast, tumors increased 14% in the control group (RPMI 1640) and 47% in groups treated with MSC-DCs. The results showed that DC therapy could target and be specific to BCSCs. DCs primed with MSCs could trigger tumor growth. These results also indicate that DCs may be a promising therapy for treating drug-resistant cancer cells as well as cancer stem cells.
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An investigation into the gap between teachers’ teaching styles and learners’ Learning styles at school of foreign languages, Thai nguyen university = Tìm hiểu khoảng trống trong phong cách giảng dậy của giảng viên và phong cách học tập của sinh viên tại khoa ngoại ngữ, Đại học Thái Nguyên .M.A. Thesis. Khoa học giáo dục: 601401
http://repository.vnu.edu.vn/handle/VNU_123/59685
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Lê triều Trung hưng công nghiệp thực lục 黎朝帝王中興功業實錄 Bản chép tay, tổng cộng 91 trang, do Đặng Phùng Xuân viết. Sách được soạn năm Vĩnh Tr...
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http://repository.vnu.edu.vn/handle/VNU_123/43569
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